Toxicokinetics of copper and cadmium in the soil model Enchytraeus crypticus (Oligochaeta)

Toxicokinetics information is key to understanding the underlying intoxication processes, although this often lacking. Hence, in present study toxicokinetics of copper (Cu) and cadmium (Cd) was assessed soil invertebrate Enchytraeus crypticus. The animals were exposed LUFA 2.2 natural spiked estimated EC20 for reproduction effects Enchytraeid Reproduction Test (ERT), i.e. 80 mg Cu/kg Dry Weight (DW) 20 Cd/kg DW. Tests followed OECD guideline 317, including a 14-day uptake phase by 14 days elimination clean soil, with samplings at 0, 1, 2, 4, 7, 10, 14. Exposure Cu showed fast uptake, reaching steady state after approx. 7 days, whereas Cd, internal concentration increased did not reach clear even days. When transferred rapidly eliminated returning initial levels, while Cd-exposed still contained residue levels These differences have consequences toxicity toxicodynamics are indicative way essential non-essential elements handled enchytraeids, likely also other invertebrates. This argues relevancy longer exposure testing like Cd compared Cu, where phenotypical can well occur later non-tested periods, e.g. 21 days’ duration standard ERT using E.